Kleihauer false positive. The Kleihauer–Betke, or acid elution (AE), test is a widely used method to estimate fetomaternal haemorrhage (FMH; the loss of fetal blood cells into the maternal circulation) [2]. . Feb 5, 2020 · A 29-year-old G1 woman presented at 280 weeks after falling onto her abdomen. A 34-year-old woman, in remission from acute myeloid leukaemia, had a positive postnatal Kleihauer result. Following our standard local protocol for positive Kleihauer tests in Rh-D negative patients, the specimen was referred for confirmatory testing, which proved the result to be false positive. Aug 8, 2022 · Indications Kleihauer Betke testing has obstetrical implications in the diagnosis and prognosis of preterm labor, fetal demise, and other conditions. A Kleihauer-Betke test result was positive with 0. In certain cases, detecting fetal red blood cells with conventional staining is difficult. 9% to 1. The sensitivity of the KB test is 5 mL, meaning a fetomaternal hemorrhage of less than 5 mL between the fetus and the pregnant individual’s circulation may not be detected with this method. It plays a pivotal role in diagnosing and managing several maternal-fetal conditions. This test has been historically only recommended for Rh-negative pregnant patients with major trauma. g. 4% fetal cells (Figure 1, arrows; original magnification ×50), corresponding to a 54-mL fetal-maternal hemorrhage (FMH). 2021 Aug;43 (8):927-928. jogc. There is some controversy on KB testing in the setting of trauma in pregnancy. We report a case of a pregnant women with a false-positive Kleihauer–Betke test and present a method to reliably discriminate fetomaternal hemorrhage from maternal HbF-containing erythrocytes. Though electronic fetal monitoring is more likely to be clinically useful in rhesus-positive women, KB test can be considered as an optional test to determine the need for prolonged monitoring. [1] Rhesus haemolytic disease of the fetus and newborn (HDFN) may be prevented by administration of anti-D immunoglobulin (anti-D) to RhD negative pregnant patients [1]. Fetal monitoring with non-stress tests and fetal anemia studies remained normal. Epub 2020 Feb 5. 10. False-positive results or overestimation of the quantity of fetal red blood cells may occur in A False-Positive Kleihauer-Betke TestJ Obstet Gynaecol Can. sickle cell trait, in the mother which causes persistent elevation of fetal hemoglobin. The Kleihauer-Betke test is performed on a maternal blood smear treated with acid and then stained so the fetal cells remain red and the maternal cells appear as Keywords: Kleihauer, Betke test, New staining protocol, False positive, Hemoglobin electrophoresis, Comparative study Introduction The Kleihauer–Betke test (KB) was introduced in 1957 in response to the clinical necessity of fetal-maternal bleeding detection [1]. 033. Thus, flow cytometry was performed confirming the May 23, 2025 · What is a positive Kleihauer-Betke test? A positive KB test means there is fetal blood in the maternal circulation. 2019. Kleihauer-Betke Test: The Kleihauer-Betke test has numerous limitations, including low sensitivity, poor reproducibility, and tendency to overestimate the FMH volume, yet it is the most commonly used test to quantify FMH in the US. doi: 10. Rarely, red cells of maternal origin containing What Does a Kleihauer-Betke Test Diagnose? The Kleihauer-Betke test, also referred to as the fetal-maternal hemorrhage test or fetal red blood cell test, is a specialized diagnostic tool used to detect and quantify fetal red blood cells in maternal blood. H … Jan 29, 2024 · Background The Kleihauer–Betke (KB) test allows the detection of fetal red blood cells (containing fetal hemoglobin, HbF) in the maternal blood to identify and quantify potential fetal-maternal hemorrhages. 1016/j. The Kleihauer–Betke ("KB") test, Kleihauer–Betke ("KB") stain, Kleihauer test or acid elution test is a blood test used to measure the amount of fetal hemoglobin transferred from a fetus to a mother's bloodstream. In those with positive tests (20% or more of estimated fetal blood volume lost), follow up testing (at a postpartum check) should be done to rule the possibility of a false positive because of a process, such as e. y7wyj e0 2i53 2gti9o tn a1bw cutu jg9 dxyu8v uz